New Features of NCS1.4 and v2 kits

 

NextSeq System Suite v1.4

The NextSeq System Suite v1.4 includes updates to the NextSeq Control Software, RTA2, NextSeq Service Software, NextSeq recipes, and the BaseSpace Broker. Changes include support for v2 reagent kits. Version 1.4 enables BaseSpace run monitoring, reagent purging, and an updated RTA quality model (Q-table) for generating Q-scores for v2 reagents. NCS v1.4 is NOT compatible with v1 reagents. Please notify us if you are planning to use v1 reagents in your sequencing run.

 

New features:

• Added support for High Output v2, Mid Output v2, and Illumina Advantage reagent kits.

• Added support for BaseSpace run monitoring while in standalone instrument mode. The feature is defaulted to off and can be enabled on the Manage Instrument > System Configuration > Analysis screen.

• Added a reagent purge feature to automatically purge all unspent reagents in the reagent cartridge to waste at the end of a sequencing run before starting the postrun wash. This feature is enabled by default and can be configured under the System Customization > Run Customization screen. Additionally, the system-wide setting can be overridden on a per-run basis from the Run Setup screen. The amount to pump is the difference between the total fill volumes and the amount that was tracked as pumped from each reagent well. Customers running 2x151 cycles sequencing runs should not see any changes as all of the reagents are consumed.

• User can now set the software to automatically advance to sequencing when the Pre-Run checks have completed successfully without a failure. This feature is defaulted to “off” and can be enabled in the System Customization screen > Run Customization screen.

• Added a wash selection screen where the user can choose to perform either a Quick Wash or a Manual Post-Run Wash.

• Run Set Up Cycles Verification.

• Added Cancel/Retry option to the Pre-Run checks.

• Software can now resume loading the reagent cartridge and flow cell consumables after closing the front door if the front door is opened while loading the consumables.

• Added cancel wash button to wash screen.

• Changed the line colors for the Corrected and Called Intensity Plot on the Sequencing Screen to match what would show up in SAV due to the latest changes in RTA.

• The update available icon is cleared when a user runs the latest system suite installer. This clears the update icon in a case where the user has installed the software update outside of NCS. 

• Added the serial number, part number, and expiration date of each consumable to the RunParameters.xml file that is saved to the run folder for each run.

• Added RunParametersVersion to RunParameters.xml to be used by consumers of RunParameters.xml to know if they can parse the file the same way. 

 

 

NextSeq v2 Reagent Kits

NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the desktop, now with improved data quality and the highest yield of error-free reads. These kits offer:

 

• Robust base calling and improved signal to noise ratio

• Multiple options for both sequencing output and read length

• Simplified reagent cartridge configurations, with improved workflow and cartridge loading

• Intuitive labeling and RFID-encoded reagents

• Optimized chemistry with the latest in SBS and bridge-amplification cluster generation

 

 

NextSeq reagent kits recommendations on different applications: