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Exome Capture Sequencing
Exome capture sequencing or exome sequencing is an NGS technique used to sequence all protein-encoding genes in a genome. There are 180,000 exons, which constitute about only 1% of the human genome with approximately 30M base pairs. However, despite of its small portion compared with the entire genome, mutations in these sequences are much more likely to induce a functionally critical alternation than in the remaining non-coding sequences and it accounts for ~85% of known disease-causing variants. Hence this sequencing approach is relatively cost-effective than commencing a whole-genome sequencing in population genetics, genetic disease or oncological studies.
We are adopting human whole exome capturing mechanism from Roche Nimblegen based on hybridization of gDNA library to SeqCap EZ oligo pool. The system provides the most comprehensive coverage of coding regaions, including coverage of genes from RefSeq RefGene CDS, CCDS, and miRBase v14 databases, and coverage of 97% Vega, 97% Gencode, and 99% Ensembl databases. Customized capturing is also available according to your experimental needs.
With exome capture and NGS, the mutational changes associated with cancer drug treatment can be identified to evaluate the acquired resistance to cancer therapy.
Whole-exome sequencing is applied to identify frequent alterations in genes involved in sister chromatid cohesion and segregation.
Exome capture sequencing can be applied in crop genotyp-phenotype relationship studies. The sequence information helps to identify mutations that could bring a fitness benefit to the plant.