Illumina NextSeq500

 

 

The most flexible desktop sequencer just got better

The NextSeq 500 desktop sequencing system provides the flexible power and simplicity you need to analyze whole genomes, exomes, and transcriptomes. The new NextSeq 500 v2 reagent kit comprising optimized sequencing reagents and improved clustering chemistry yields a high data quality.

 

Performance

Read Length:       Maximum ~150 bases

Throughput:        16.25-19.5Gb (2 x 75bp, MID OUTPUT KIT) to

                              100-120Gb (2 x 150bp, HIGH OUTPUT KIT)

Reads per Run:   Up to 400M single reads; 800M paired-end reads

Accuracy:             >80% bases higher than Q30 at 1 x 75bp, HIGH OUTPUT KIT;

                              >80% bases higher than Q30 at 2 x 75bp, HIGH OUTPUT KIT;

                              >75% bases higher than Q30 at 2 x 150bp, HIGH OUTPUT KIT;

                              >80% bases higher than Q30 at 2 x 75bp, MID OUTPUT KIT;

                              >75% bases higher than Q30 at 2 x 150bp, MID OUTPUT KIT

Run Time:            ~11 hours (1 x 75bp) to ~29 hours (2 x 150bp)

 

Applications

Targeted Resequencing

Small-Genome Sequencing

RNA Sequencing

Quality Control

Regulation