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Bisulfite Sequencing
DNA methylation at cytosine nucleotides (forming 5-methylcytosine, 5mC) is a critical epigenetic component in regulating various spatial and temporal cellular processes. The methylation affects gene expression, chromatin remodeling, cell differentiation and suppression of transposable elements which in turn influence health and development of the organism. NGS technology enables genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Two options of bisulfite sequencing method are available: whole-genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS). WGBS provides information on all methylation sites throughout the genome, while RRBS covers >85% CpG islands and is an cost-effective alternative for detecting cytosine modifications.
With exome capture and NGS, the mutational changes associated with cancer drug treatment can be identified to evaluate the acquired resistance to cancer therapy.
Bisulfite sequencing applied on investigation of the effect of epigenomic alternations on a lethal infant disease.
Single-cell bisulfite sequencing (scBS-Seq) assists to explore DNA methylation in rare cells and heterogeneous populations
Different methylation detection methods accommodate different requirements on the depth of coverage in polymorphic methylation patterns.