Amplicon Sequencing

 

Amplicon Sequencing, with targeted amplicons of interest, such as 16S rDNA or other phylogenetically informative sequences for microbial diversity studies, marker genes for cancer or genetic disease identification and characterization, etc., allows for ultra-deep highly sensitive detection of target amplicons of choices unbiasedly. Direct PCR products resulting from PCR with library adaptors and multiplex barcode linker-fusion primers speed up sample sequencing without the need for intricate cloning and library preparation steps. Our Illumina MiSeq platform has notable advantages in multiplexing large number of samples for high-throughput amplicon sequencing. Amplicon variance analyses are popular for the study of population genetics, genetic diseases detection, methylation patterns and metagenomes.

 

With the high throughput and multiplex capacity of NGS platforms, multi-locus and multi-taxa phylogenetics or detection of genetic mutation or marker genes are particularly advantageous and beneficial to comparative genomic and clinical diagnostic applications.